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Home Lab Consumables >TPP Plasticware>TUBESPIN® BIOREACTOR>TUBESPIN® BIOREACTOR
TUBESPIN® BIOREACTOR Maximize

TUBESPIN® BIOREACTOR

Accelerate the process development for a new therapeutic protein

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TubeSpin® bioreactors are ideal for large-scale screenings and optimizing of suspension cell system process developments. The important parameters for the production upscaling can be evaluated with small amounts. This lowers the cost and increases the efficiency of the process.

Features:

TubeSpin® Bioreactor

  • TubeSpin® Bioreactor 15: working volume 1 – 15 ml 2 versions available: round or conical bottom
  • TubeSpin® Bioreactor 50: working volume 1 – 35 ml
  • TubeSpin® Bioreactor 450: working volume up to approx. 300 ml
  • TubeSpin® Bioreactor 600: working volume up to approx. 400 ml
  • Filter screw cap with partly 5 different openings (A-B-C-D-E)
  • Size of openings-
    • 87015 + 87017: 5 x openings: A = 0.4 mm, B = 0.6 mm, C = 1.0 mm, D = 1.25 mm, E = 1.5 mm
    • 87050: 5 x openings:  A = 0.4 mm, B = 0.6 mm, C = 1.0 mm, D = 1.5 mm, E = 2.0 mm
    • 87450/87600: 10 x openings with size 4.0 mm each
  • Fit standard centrifuge apparatus
  • Spin: 15,500 gx or 3,500 gx
  • Matching adapter plates for shakers for cultivation of suspension cells in a TubeSpin® Bioreactor 600 available

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Product-No. Version Centrifugal Acceleration max gx Volume ml Dimensions Ø x h mm Material Qty/ Bag pcs Qty/ Case pcs
87015 conical 15,500 15 17.1 x 120 PP 40 800
87017 round 15,500 15 17.1 x 120 PP 40 800
787050 conical, barcode 15,500 50 30.0 x 115 PP 20 180
87050 conical 15,500 50 30.0 x 115 PP 20 180
87450 conical 3,500 450 96 x 145 PP 1 32
87600 conical 3,500 600 100 x 183 PP 1 26
 
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Rack and consumables for TubeSpin® bioreactors   
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Product-No. Version Capacity pcs Dimensions l x w x h mm Material Qty/ Bag pcs Qty/ Case pcs
87355 Screw cap "Filter" for 450/600 - 70 x 21 PE 1 20
87356 Screw cap "solid" for 450/600 - 70 x 21 PE 1 20
99013 Rack for 87450/87600 3 x 450 ml / 600 ml 366 x 146 x 70 PP 1 6
99017 Rack "S" for 87015/87017/87050 18 x 15 ml / 10 x 50 ml 209 x 98 x 62 PP 1 45

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"Dr. Shaker" offers advice on any issues concerning shaken bioreactors. His recommendations for spin, throw and shaking speed are

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Mammalian Cell culture Insect Cells Plant Cell culture
TubeSpin® 50 CHO HEK-293 SF-9 N. tabacum BY-2
Shaking Speed [rpm] 180 -  250 180 - 250 180 - 250 180 - 250
Throw [mm] 50 50 50 50
Working Volume [mL] 15 - 30 15 - 30 5 - 10 5 - 10
 
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Mammalian Cell culture Insect Cells Plant Cell culture
TubeSpin® 600 CHO HEK-293 SF-9 N. tabacum BY-2
Shaking Speed [rpm] 180 - 250 180 - 250 180 -  250 180 - 250
Throw [mm] 50 50 50 50
Working Volume [mL] 300 300 200 150
  Methods from Literature
TubeSpin® 50 CHO CHO CHO CHO SF-9
Shaking Speed [rpm] 180-300 160-200 140 200 160-200
Throw [mm] 50 n.s. 50 25 n.s.
Working Volume [mL] 14-34 10-35 5-10 5 10-35
Reference Strnad (2010), Biotechnol. Prog.: Vol. 26 (3); pp 653-663   Xie (2011), Cytotechnolog y: Vol. 63 (4); pp 345-350 Stettler (2007), Biotechnol. Prog.: Vol. 23 (6); pp 1340 -1346 De Jesus (2004), Biochem. Eng. Journal: Vol 17, No. 3; pp 217 - 223 Shen (2011), BMC Proc.: 5 (Suppl 8); P37
TubeSpin® 600 CHO Crypthecodinium cohnii (marine microalgae)
Shaking Speed [rpm] 120-220 230
Throw [mm] 50 25
Working Volume [mL] 300 100
Reference Monteil (2013), Biochem. Eng. Journal; Vol. 76, pp 6–12 Hillig (2014), Adv Biochem Eng Biotechnol; Vol. 138: pp 179-206
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Ideal volume of culture medium for use in TPP TubeSpin® bioreactor

Volume recommendations must be seen as guideline and do not represent an absolute value. TPP is not responsible for any outcome in this regard. The usual media volume to surface area is 0.20 - 0.5ml/cm2, the upper limit is set by gaseous diffusion through the liquid layer and the optimum will depend upon the oxygen requirement of the cells. Also, more confluent cultures require more media and changing more frequently.

The number of cells on a confluent plate, dish, or flask will vary with cell type. For this table, we give no recommendations, but indicate source of information such as: Freshny R. Culture of animal cells Wiley-Liss 1994, etc.

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Product No. Max Volume  (ml) Shaker Ø mm / speed rpm Medium recom. (ml) Medium max. (ml)
86050 50 50 / 250 35 50
87015 15 50 / 250 10 15
87017 15 50 / 180 10 15
87050 50 50 / 150 35 50
87450 450 50 / 150 250 450
87600 600 50 / 150 400 600