Maximize
TISSUE CULTURE FLASKS 25 – 300 CM2
Homely environment for your cells.
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8 other products in the same category
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Features:
- Surface activated growth area for optimal adhesion and best proliferation of adherent cells
- Geometry of the neck reduces risk of media slop over
- Staple rim offers stable, slip free stapling of several flasks
- Highly visible bilateral marking areas
- White volume graduations on both sides for optic control of the filling volume
- Crystal clear transparency
| Product-No. | Growth Area cm | Version | Volume max. ml | Volume recom. ml | Dimensions l x w x h mm | Material | Qty/ Bag pcs | Qty/ Case pcs |
| 90025 | 25 | VENT | 15 | 3-8 | 92 x 51 x 29 | PS | 10 | 360 |
| 90026 | 25 | Filter | 15 | 3-8 | 92 x 51 x 29 | PS | 10 | 360 |
| 90075 | 75 | VENT | 65 | 8-22 | 155 x 87 x 40 | PS | 5 | 100 |
| 90076 | 75 | Filter | 65 | 8-22 | 155 x 87 x 40 | PS | 5 | 100 |
| 90150 | 150 | VENT | 165 | 15-45 | 210 x 122 x 50 | PS | 3 | 36 |
| 90151 | 150 | Filter | 165 | 15-45 | 210 x 122 x 50 | PS | 3 | 36 |
| 90300 | 300 | VENT | 410 | 30-85 | 275 x 170 x 50 | PS | 3 | 18 |
| 90301 | 300 | Filter | 410 | 30-85 | 275 x 170 x 50 | PS | 3 | 18 |
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Ideal volume of culture medium for use in TPP tissue culture flasks
Volume recommendations must be seen as guideline and do not represent an absolute value. TPP is not responsible for any outcome in this regard. The usual media volume to surface area is 0.20 - 0.5ml/cm2, the upper limit is set by gaseous diffusion through the liquid layer and the optimum will depend upon the oxygen requirement of the cells. Also, more confluent cultures require more media and changing more frequently.
The number of cells on a confluent plate, dish, or flask will vary with cell type. For this table, we give no recommendations, but indicate source of information such as: Freshny R. Culture of animal cells Wiley-Liss 1994, etc.
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| Product No. | Version | Growth area (cm2) | Medium recom. (ml) | Medium max. (ml) |
| 90025 | VENT | 25 | 3 - 5 | 15 |
| 90026 | Filter | |||
| 90075 | VENT | 75 | 8 - 15 | 65 |
| 90076 | Filter | |||
| 90150 | VENT | 150 | 15 - 30 | 165 |
| 90151 | Filter | |||
| 90300 | VENT | 300 | 30 - 50 | 410 |
| 90301 | Filter |